Is repeated cypermethrin fumigation dangerous for the mitochondrial DNA in dry insect samples?

Vondráček Dominik, Tkoč Michal, Fikáček Martin
Acta Entomologica Musei Nationalis Pragae 58(2): 609-614, 2018
Published online: 14th December 2018
Published in print: 31st December 2018
Views: 1194
Abstract: Entomological collections are the target of various insect pests, e.g. carpet beetles (Dermestidae) and booklice (Psocoptera) which can damage and completely destroy dry specimens in a relatively short time. Collections in the National Museum, Czech Republic (NMP) including the entomological ones are protected by fumigation using commercially available smoke shells ‘Cytrol Super SG’; fumigation is performed twice a year. The active insecticidal substance of these smoke shells is cypermethrin (6.25%). We tested whether the repeated cypermethrin fumigation of the NMP entomological collections negatively affects the quality of mitochondrial DNA in dry specimens and prevents the subsequent use of these samples for molecular analyses required for identification, taxonomy, systematics, and phylogenetic studies. We used 32 freshly fixed specimens of the flower chafer Oxythyrea funesta (Poda von Neuhaus, 1761) and 32 freshly fixed specimens of the brown-tailed cockroach Supella longipalpa (Fabricius, 1798). One half of specimens of both species was stored outside NMP and not fumigated (negative control), and the other half was deposited in collection hall with the NMP insect collection and directly exposed to the fumigation. Subsequently, all specimens were processed in a molecular laboratory under a standardized protocol using one leg as the source tissue after each fumigation, and the 658 bp long barcoding region of the cytochrome oxidase I (cox1) as the testing gene fragment. Results of the PCR product electrophoresis and the sequences acquired confirmed that the repeated fumigation had no negative effect on tested samples.
Key words: Entomological collections, pest protection, fumigation, cypermethrin, molecular analysis, DNA quality, cytochrome oxidase I
Taxonomic revision of the genus Glenopopillia (Coleoptera: Scarabaeidae: Rutelinae)Cuccodorodes gen. nov., an apterous Batrisitae from the tropical Himalayas (Coleoptera: Staphylinidae: Pselaphinae)Taxonomic review of the genus Calyptopsis of Turkey and adjacent areas (Coleoptera: Tenebrionidae: Tentyriini)Taxonomic notes on the genus Hydrobaenus with description of H. simferopolus sp. nov. from Crimea (Diptera: Chironomidae)Review of the plant bug genus Prolygus and related mirine taxa from eastern Asia (Hemiptera: Heteroptera: Miridae)Catalogue of the Incini with the description of the first Archedinus species from Honduras (Coleoptera: Scarabaeidae: Cetoniinae)Synapsis puluongensis sp. nov. and redescription of S. horaki (Coleoptera: Scarabaeidae), with a key to Vietnamese speciesRevision of the subgenus Nessus of the genus Hypocaccus from Central Europe, with description of a new species (Coleoptera: Histeridae)The last instar larva and pupa of Ora depressa (Coleoptera: Scirtidae), a marsh beetle with underwater pupationEleven new species and a new country record of Pselaphodes (Coleoptera: Staphylinidae: Pselaphinae) from China, with a revised checklist of world speciesTaxonomic review of Chin-wen Chen’s species described in the genus Smicromyrme (Hymenoptera: Mutillidae)A new species of genus Oxytauchira from India (Orthoptera: Acrididae)Larval chaetotaxy and morphometry of Oosternum costatum (Coleoptera: Hydrophilidae) including a discussion of larval characters with phylogenetic relevanceSelasia dembickyi sp. nov., the first member of Drilini (Coleoptera: Elateridae) from South East Asia, with the description of S. jenisi sp. nov. from NepalDescription of a new species of Rhizococcus (Hemiptera: Coccomorpha: Acanthococcidae) from IranThe Unicorn exists! A remarkable new genus and species of Perilissini (Hymenoptera: Ichneumonidae) from South East AsiaNew minute Drilini species significantly extend the distributions of Lolosia and Microselasia (Coleoptera: Elateridae: Agrypninae) in tropical AfricaA new species of the genus Nenasa (Hemiptera: Fulgoroidea: Caliscelidae) from mainland ChinaA new species and two new synonyms of Mongolian Nemotelus (Diptera: Stratiomyidae)A new species of the genus Paratrichius from northern Vietnam (Coleoptera: Scarabaeidae: Cetoniinae)Two species previously placed in Euconnus s. str. belong in the endemic Madagascan subgenus Glabriconnus (Coleoptera: Staphylinidae: Scydmaeninae)Gibbocicada brasiliana, new genus and new species from Brazil and a key for the genera of Tibicinini (Hemiptera: Auchenorrhyncha: Cicadidae)Two new species of Glischrochilus with taxonomic comments, new records from Asia, and a world checklist of the genus (Coleoptera: Nitidulidae)New species of bristletails of the genus Lepismachilis (Archaeognatha: Machilidae) from Serbia and MontenegroSix new species of Dolichopeza (Nesopeza) from China (Diptera: Tipulidae)Is repeated cypermethrin fumigation dangerous for the mitochondrial DNA in dry insect samples?