DNA barcoding applied: identifying the larva of Merodon avidus (Diptera: Syrphidae)

Andrić Andrijana, Šikoparija Branko, Obreht Dragana, Đan Mihajla, Preradović Jelena, Radenković Snežana, Perez-Bañon Celeste, Vujić Ante
Acta Entomologica Musei Nationalis Pragae 54(2): 741-757, 2014
Published in print: 15th December 2014
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Abstract: The immature stages of phytophagous hoverflies and their plant hosts are mostly unknown. This paper describes the larva of Merodon avidus Rossi, 1790 (Diptera: Syrphidae), based on material collected from Đerdap National Park in Serbia. Larvae were found in the bulbs of Ornithogalum L. (Hyacinthaceae) and in the surrounding soil. DNA barcoding was used to identify the species and scanning electron microscopy was used to describe the morphological characteristics of Merodon avidus. Reared material previously identified as Merodon constans (Rossi, 1794) is shown to belong to M. hurkmansi Marcos-García, Vujić & Mengual, 2007.
Key words: Diptera, Syrphidae, Merodon, hoverflies, immature stages, larval morphology, DNA barcoding, Serbia, Palaearctic Region
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